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KMID : 0381120150370070607
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Genes and Genomics
2015 Volume.37 No. 7 p.607 ~ p.614
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Genes related with apoptosis by inflammation in diabetic keratocytes
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Park Young-Min
Lee Ji-Eun
Kim Chi-Dae
Lee Jong-Soo
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Abstract
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The goal of this study was to identify the differences in apoptosis-related gene expression between normal and diabetic keratocytes stimulated with IL-1α and TNF-α. Primary cultured normal and diabetic keratocytes were treated with IL-1α and TNF-α. The cDNA from these cells was hybridized to an oligonucleotide microarray. Microarray analysis was used to identify differentially expressed genes, which were further evaluated by real-time polymerase chain reaction. Diabetic keratocytes overexpressed vital components of cell division, migration, and differentiation, including Ppm1a, Ncam1, and Capn9, and under-expressed components related to prevention of apoptosis, including Clu, Ptn, and Mycn. Genes commonly over-expressed in response to IL-1α and TNF-α include Siah1a, Gzmb, and Capn9. Genes underexpressed after treatment with both cytokine include Ets1, Igf1r, and Sgk. Eighteen and eight apoptosis-related genes were newly expressed after treatment with IL-1α and TNF-α, respectively. The genes commonly under-expressed after treatment with both cytokines were Bmp4 and Timp3. Real-time polymerase chain reaction showed results similar to those of the microarray analysis. IL-1α and TNF-α may modulate the expression of genes related to apoptosis in diabetic keratocytes. It may be helpful to suppress newly overexpressed genes or to enhance newly underexpressed genes by IL-1α or TNF-α for the prevention of apoptosis in keratocytes when the cornea is damaged.
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KEYWORD
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Apoptosis, IL-1α, Inflammation, Keratocyte, Microarray, TNF-α
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